Composite
Part:BBa_K1827001:Design
Designed by: Yuree Chung Group: iGEM15_HAFS-Korea (2015-09-14)
Trichoderma reesei bgl1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 266
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 751
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 337
Illegal NgoMIV site found at 557
Illegal NgoMIV site found at 1045 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 975
Illegal SapI.rc site found at 1359
Design Notes
No additional considerations were necessary since the designed sequence consists of the whole bgl1 gene.
Source
The gene is a part of the genome of trichoderma reesei. It is obtained through direct DNA extraction method.